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Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 μg/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate. SD8837, SD8839, SD8841, SD8842, SD8843, and negative control sdAb 1H4 at decreasing concentrations.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) SD8837, (B) SD8839, (C) SD8841, (D) SD8842, (E) SD8843, and (F) SD8733 negative control sdAb. Single domain antibodies at 5 μg/ml.
Immunohistochemistry of LAG-3 in human lymph node tissue using (A) SD8837, (B) SD8839, (C) SD8841, (D) SD8842, (E) SD8843, and (F) SD8733 negative control sdAb. Single domain antibodies at 1 μg/ml.
Flow cytometry analysis of LAG-3 in over expressing HEK293 cells using (A) SD8837, (B) SD8839, (C) SD8841, (D) SD8842, (E) SD8843, and (F) Negative control SD8733. Single domain antibodies at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 μg/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate. SD8837, SD8839, SD8841, SD8842, SD8843, and negative control sdAb 1H4 at decreasing concentrations.

LAG3 Detection Set

CATALOG NUMBER: SD8700

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P
Host Species:
Llama
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Llama
Species Reactivity:
Human
Immunogen:
LAG3 antibodies were raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P
Set Contents:
20 μg:
LAG-3 Antibody [2A11], Cat. No. SD8837
LAG-3 Antibody [1A6], Cat. No. SD8839
LAG-3 Antibody [2B11], Cat. No. SD8841
LAG-3 Antibody [1C6], Cat. No. SD8842

100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669
Purification:
LAG3 Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein.  They also contains a myc-tag for detection.
Clonality:
Monoclonal
Isotype:
sdab
physical-state:
Liquid
Buffer:
LAG3 Antibodies are supplied in PBS.
Concentration:
Antibody 1 mg/mL
Storage Conditions:
LAG3 antibodies should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
Background Reference 1:
Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
Background Reference 2:
Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
Background Reference 3:
Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
Background Reference 4:
Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.
Set Contents:
20 μg:
LAG-3 Antibody [2A11], Cat. No. SD8837
LAG-3 Antibody [1A6], Cat. No. SD8839
LAG-3 Antibody [2B11], Cat. No. SD8841
LAG-3 Antibody [1C6], Cat. No. SD8842

100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

SD8700

List Size:
1 Set

List Price:

$429.00

Datasheet
Shipping Info

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