Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of PD-L2 in overexpressing 293 cells using RF16021, RF16022, RF16023, RF16024, and RF16025 antibody at 0.5 and 1 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2.
Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 5 μg/ml.
Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 μg/ml.
Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 μg/ml.
Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.
Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with RF16021, RF16022, RF16023, RF16024, and RF16025 abs at decreasing concentrations.
Immunofluorescence of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 μg/ml.
Immunohistochemistry of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 μg/ml.
Western blot analysis of PD-L2 in overexpressing 293 cells using RF16021, RF16022, RF16023, RF16024, and RF16025 antibody at 0.5 and 1 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2.

PDL2 Detection Set (Risk Free)

CATALOG NUMBER: RF16020

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Mouse monoclonal PD-L2 antibodies were raised against the extracellular domain of human PD-L2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P, WB
Set Contents:
PD-L2 Antibody [4E10], Cat. No. RF16021 (20 μg)

PD-L2 Antibody [8C12], Cat. No. RF16022 (20 μg)

PD-L2 Antibody [7C7], Cat. No. RF16023 (20 μg)

PD-L2 Antibody [7C1], Cat. No. RF16024 (20 μg)

PD-L2 Antibody [10H4], Cat. No. RF16025 (20 μg)
Positive Control 1:
Cat. No. RF10001-06 - Raji Lysate
Positive Control 2:
Cat. No. RF10001-04 - Human Tonsil Tissue Lysate
Purification:
Antibodies are supplied as protein A purified IgG1.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Storage Conditions:
Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through an alternative receptor from PD-1.ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
Background Reference 1:
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Background Reference 2:
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
Background Reference 3:
LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Background Reference 4:
Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.
Set Contents:
PD-L2 Antibody [4E10], Cat. No. RF16021 (20 μg)

PD-L2 Antibody [8C12], Cat. No. RF16022 (20 μg)

PD-L2 Antibody [7C7], Cat. No. RF16023 (20 μg)

PD-L2 Antibody [7C1], Cat. No. RF16024 (20 μg)

PD-L2 Antibody [10H4], Cat. No. RF16025 (20 μg)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

RF16020

List Size:
1 Set

List Price:

$509.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369