Goat Anti-Mouse IgG (H&L) Antibody (biotin)

CATALOG NUMBER: XR-9740

Tested Applications:
ELISA
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
Mouse IgG - whole molecule
Conjugate:
Biotin
Tested Applications:
ELISA
Application Note:
Biotin Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).
Specificity:
Minimum Cross reactivity to Human Serum Proteins
Purification:
Biotin Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1.0% cross reactivity against human IgG.
Isotype:
IgG
physical-state:
Lyophilized
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
Storage Conditions:
Biotin Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4 °C prior to restoration. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XR-9740

List Size:
2 mg

List Price:

$334.00

Shipping Info
Customize your order
Custom Antibody Services

New & Featured Products

CATALOG NUMBER: PM-7369-HRP

CATALOG NUMBER: PM-7369-biotin

CATALOG NUMBER: PM-7369