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Human CD31, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human CD31, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

PECAM1 Recombinant Protein

CATALOG NUMBER: 96-093

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln 28 - Lys 601
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
66.4 kDa
Biological Activity:
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 50000 cells/well are added to rhCD31 coated plates (3. 3 μg/mL, 100 μl/well) in the presence of 20 ng/mL PMA, approximately 55 - 65% will adhere after 30 minutes at 37°C.
Purity:
>95% as determined by SDS-PAGE.

Less than 1.0 EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
50 mM Tris, 150 mM NaCl, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
PECAM1
Additional Names:
PECAM1, CD31, FLJ34100, FLJ58394
Protein Accession Number:
P16284-1
Ncbi Gene Id Number:
5175
Background:
Cluster of Differentiation 31 (CD31) is also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.
Background Reference 1:
Newman PJ, Newman DK., 2004, Arterioscler. Thromb. Vasc. Biol. 23 (6): 953–64.
Background Reference 2:
Kalinowska A, Losy J., 2007, Eur. J. Neurol. 13 (12): 1284–90.
Background Reference 3:
Almendro N, et al., 2007, J. Immunol. 157 (12): 5411–21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-093

List Size:
0.1 mg

List Price:

$463.00

Datasheet
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