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Human HMGB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human HMGB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HMGB1 Recombinant Protein

CATALOG NUMBER: 96-393

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Met1 - Glu 215
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
25.7 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA Immobilized human HMGB at 2μg/ml (100μl/well) can bind human AGER. The EC50 of human AGER is 0. 22μg/ml.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
HMGB1
Additional Names:
HMGB1, HMG1, HMG3, SBP-1
Protein Accession Number:
NP_002119.1
Ncbi Gene Id Number:
3146
Background:
High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
Background Reference 1:
Wang H, et al., 1999, Science 285 (5425): 248–51.
Background Reference 2:
Yang H, et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107 (26): 11942–7.
Background Reference 3:
Yang H, et al., 2010, Biochim. Biophys. Acta 1799 (1-2): 149–56.
Background Reference 4:
Fagone P, et al., 2011, Gene Ther. 18 (11): 1070–7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-393

List Size:
0.1 mg

List Price:

$626.00

Datasheet
Shipping Info

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