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The purity of rh LIF was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh LIF was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

LIF Recombinant Protein (HEK293 Expressed)

CATALOG NUMBER: 96-519

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ser 23 - Phe 202
Fusion Tag:
Tag free
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
19.9 kDa
Biological Activity:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0. 1 ng/mL, corresponding to a specific activity of >1X107 Unit/mg.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
LIF
Additional Names:
LIF, CDF, DIA, HILDA, MLPLI
Protein Accession Number:
AAH69540.1
Ncbi Gene Id Number:
3976
Background:
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Background Reference 1:
Wenwei Hu, Zhaohui Feng, Angelika K. Teresky1, Arnold J. Levine (November 29, 2007).
Background Reference 2:
Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L (2009). PLoS ONE 4 (7): e6343.
Background Reference 3:
Patterson PH (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (17).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-519

List Size:
0.02 mg

List Price:

$365.00

Datasheet
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