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The purity of rh PDL1 /CD274,twin strep tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh PDL1 /CD274,twin strep tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

PDL1 Recombinant Protein

CATALOG NUMBER: 96-599

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Phe 19 - Arg 238
Fusion Tag:
C-Twin Strep Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
28.2 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Strep Tag at 5 ug/mL (100 ul/well) can bind Biotinylated Human PD-1, His Tag & Fc tag with a linear range of 2-60 ng/mL.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CD274
Additional Names:
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Protein Accession Number:
NP_054862.1
Ncbi Gene Id Number:
29126
Background:
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Background Reference 1:
Dong H., et al., 1999, Nat. Med. 5:1365-1369.
Background Reference 2:
Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
Background Reference 3:
Nishimura H., et al., 2001, Trends in Immunology 22:265. 

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-599

List Size:
0.025 mg

List Price:

$495.00

Datasheet
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