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Human GAPDH, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human GAPDH, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

GAPDH Recombinant Protein

CATALOG NUMBER: 96-878

Tested Applications:
WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Gly 2 - Glu 335
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
36.9 kDa
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
50 mM Tris, 150 mM NaCl, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
GAPDH
Additional Names:
GAPDH,GAPD,CDABP0047,OK/SW-cl.12
Protein Accession Number:
AAH01601
Ncbi Gene Id Number:
2597
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is also known as Peptidyl-cysteine S-nitrosylase GAPDH, which is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH can participate in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Also, GAPDH can modulate the organization and assembly of the cytoskeleton. Furthermore, GAPDH is a component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.
Background Reference 1:
Tokunaga K., et al., 1987, Cancer Res. 47:5616-5619.
Background Reference 2:
Allen R.W., et al., 1987, J. Biol. Chem. 262:649-653.
Background Reference 3:
Jarczowski F., et al., 2009, J. Biol. Chem. 284:766-773.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-878

List Size:
0.1 mg

List Price:

$463.00

Datasheet
Shipping Info

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