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Unconjugated Human CD47, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Unconjugated Human CD47, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

CD47 Recombinant Protein

CATALOG NUMBER: 97-071

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln 19 - Pro 139
Fusion Tag:
His Tag & Avi Tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Predicted Molecular Weight:
16.9 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha, Fc Tag at 2 ug/mL (100 uL/well) can bind Unconjugated Human CD47, His Tag, Avi Tag with a linear range of 2-12.8 ng/mL.
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
10 mM Bicine, pH8.3
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CD47
Additional Names:
CD47,MER6,IAP,OA3
Protein Accession Number:
NP_942088
Ncbi Gene Id Number:
961
Background:
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Background Reference 1:
Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.
Background Reference 2:
Latour S., et al., 2001, J. Immunol. 167:2547-2554.
Background Reference 3:
Piccio L., et al., 2005, Blood 105:2421-2427.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

97-071

List Size:
0.05 mg

List Price:

$495.00

Datasheet
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