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Human BTLA (31-150), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human HVEM, His Tag at 5 ug/mL (100 uL/well) can bind Human BTLA (31-150), Mouse IgG2a Fc Tag with a linear range of 10-156 ng/mL (QC tested).
Human BTLA (31-150), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

BTLA (31-150) Recombinant Protein

CATALOG NUMBER: 11-243

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Lys 31 - Ser 150
Fusion Tag:
Mouse IgG Fc Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Predicted Molecular Weight:
40.6 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
100 mM Glycine, 150 mM NaCl, Arginine, pH3.2
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
BTLA
Additional Names:
BTLA,CD272
Protein Accession Number:
AAP44003.1
Ncbi Gene Id Number:
151888
Background:
B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
Background Reference 1:
Watanabe N., et al., 2003, Nat. Immunol. 4:670-679.
Background Reference 2:
Gavrieli M., et al., 2003, Biochem. Biophys. Res. Commun. 312:1236-1243.
Background Reference 3:
Sedy J.R., et al., 2005, Nat. Immunol. 6:90-98.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-243

List Size:
0.1 mg

List Price:

$577.00

Datasheet
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