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NMNAT3 highly active Recombinant Protein

CATALOG NUMBER: 90-357

Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.
Fusion Tag:
His Tag
Application Note:
This recombinant proteins is for research use only.
Biological Activity:
>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol of NAD+ per min.
Purity:
>95% (SDS-PAGE)
physical-state:
Liquid
Buffer:
In 50mM TRIS-HCl, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
Concentration:
1mg/ml
Storage Conditions:
Working aliquots are stable for up to 3 months when stored at -20°C.
Ncbi Official Symbol:
NMNAT3
Additional Names:
Nicotinamide Mononucleotide Adenylyltransferase 3, NMN Adenylyltransferase 3, NaMN Adenylyltransferase 3, EC 2.7.7.18, EC 2.7.7.1
Protein Accession Number:
Q96T66
Protein Gi Number:
30039706
Ncbi Gene Id Number:
349565
Background:
Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i. e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

90-357

List Size:
50 ug

List Price:

$682.00

Datasheet
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