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Immobilized SARS-CoV S protein RBD (N501Y), His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.
The purity of SARS-COV-2 S protein RBD (N501Y) is greater than 95% as determiend by SEC-HPLC.
Human ACE2 captured in protein A chip, can bind SARS-CoV S protein RBD (N501Y), His Tag with an affinity constant of 1.74nM as determined in SPR assap (Biacore T200).
SARS-COV2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity os great than 95%.
Immobilized SARS-CoV S protein RBD (N501Y), His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.

SARS-CoV-2 (COVID-19) UK variant (B.1.1.7) S RBD (N501Y) Recombinant Protein

CATALOG NUMBER: 11-069

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
SARS Spike Protein
source-species:
HEK293 cells
Recombinant Protein Sequence:
Arg319 - Phe541 (N501Y)
Fusion Tag:
His-Avi
Tested Applications:
ELISA, WB
Application Note:
SDS-PAGE
Purity:
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Endotoxin level is less than 1EU per ug by the LAL method.
physical-state:
Lyophilized
Buffer:
20mM PB (pH 7.4). 5 % trehalose is added as protectant before lyophilization.
Storage Conditions:
Store in working aliquots at -20°C. Avoid freeze/thaw cycles.
Additional Names:
S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Protein Accession Number:
QHD43416.1
Background:
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-069

List Size:
0.1 mg

List Price:

$515.00

Datasheet
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