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IL-2 Recombinant Protein

CATALOG NUMBER: 91-011

Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
Pro22-Thr153
Fusion Tag:
Tag Free
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Predicted Molecular Weight:
15.4 kD
Biological Activity:
ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of murine CTLL-2 cells.
Specific Activity of 1.0 x 107 IU/ mg.
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Storage Conditions:
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at -20°C for 3 months.
Ncbi Official Symbol:
IL2
Additional Names:
Interleukin-2, IL-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2
Protein Accession Number:
P60568
Ncbi Gene Id Number:
3558
Background:
Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

91-011

List Size:
0.05 mg

List Price:

$328.00

Datasheet
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