Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

SDS-PAGE analysis of recombinant EndoG fragment on Coomassie Blue-stained 4-20% gradient gel.
SDS-PAGE analysis of recombinant EndoG fragment on Coomassie Blue-stained 4-20% gradient gel.

EndoG Fragment Recombinant Protein

CATALOG NUMBER: 95-103

Tested Applications:
ELISA, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
aa. 51 - 140
Fusion Tag:
Fusion Partner: His-tag and strepII-tag at N-terminus
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
15 kDa (Calculated)
Purity:
~95%
physical-state:
Liquid
Buffer:
1X PBS containing 0.1% SDS and 0.02% NaN3
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
ENDOG
Additional Names:
EndoG Antibody : Endo G
Protein Accession Number:
Q14249
Protein Gi Number:
24638471
Ncbi Gene Id Number:
2021
Background:
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4).
Background Reference 1:
Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9.
Background Reference 2:
Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans . Nature 2001; 412:90-4
Background Reference 3:
Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29.
Background Reference 4:
Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-103

List Size:
0.05 mg

List Price:

$289.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369