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SDS-PAGE analysis of recombinant IRAK fragment on Coomassie Blue-stained 12% SDS-PAGE gel.
SDS-PAGE analysis of recombinant IRAK fragment on Coomassie Blue-stained 12% SDS-PAGE gel.

IRAK Recombinant Protein

CATALOG NUMBER: 95-112

Tested Applications:
ELISA, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
aa 618 - 712
Fusion Tag:
Fusion Partner: C-terminal His-tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
15 kDa (Calculated)
Purity:
~95%
physical-state:
Liquid
Buffer:
100mM sodium phosphate, 500mM NaCl, 25 mM imidazole, 10% gycerol
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
IRAK1
Additional Names:
IRAK Antibody: IRAK, pelle, IRAK, Interleukin-1 receptor-associated kinase 1, IRAK-1
Protein Accession Number:
P51617
Protein Gi Number:
8928535
Ncbi Gene Id Number:
3654
Background:
Nuclear factor kappa B (NF-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).
Background Reference 1:
Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
Background Reference 2:
Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.
Background Reference 3:
Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.
Background Reference 4:
Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-112

List Size:
0.05 mg

List Price:

$289.00

Datasheet
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