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Western blot analysis of RICK in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RICK antibody at 1 μg/mL.
Immunocytochemistry of RICK in A431 cells with RICK antibody at 10 μg/mL.
Immunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/ml.
Immunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/mL.<br><br>Red: RICK Antibody (2075) <br> Blue: DAPI staining
Immunocytochemistry of RICK in K562 cells with RICK antibody at 2.5 μg/mL.
Western blot analysis of RICK in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RICK antibody at 1 μg/mL.
Trial Size

RICK Antibody

CATALOG NUMBER: 2075

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
RICK antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK.
The immunogen is located within the first 50 amino acids of RICK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
RICK antibody can be used for detection of RICK by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1225 - Ramos Cell Lysate
Positive Control 3:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 4:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 5:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 6:
Cat. No. 17-002 - A-431 Cell Slide
Predicted Molecular Weight:
Predicted: 59 kDa
Observed: 59 kDa
Purification:
RICK Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RICK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
RICK antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RIPK2
Additional Names:
RICK Antibody: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, UNQ277/PRO314/PRO34092, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase
Protein Accession Number:
O43353
Protein Gi Number:
20455217
Ncbi Gene Id Number:
8767
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RICK Antibody: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β. The messenger RNA of RICK is expressed in multiple human tissues.
Background Reference 1:
Inohara N, del Peso L, Koseki T, et al. RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 1998; 273:12296-300.
Background Reference 2:
McCarthy JV, Ni J, and Dixit VM. RIP2 is a novel NF-κB-activating and cell death-inducing kinase. J. Biol. Chem. 1998; 273:16968-75.
Background Reference 3:
Thome M, Hofmann K, Burns K, et al. Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 1998; 8:885-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2075

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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