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Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.
Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 μg/mL.
Immunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 μg/ml.
Immunofluorescence of CIDE-A in human brain tissue with CIDE A antibody at 20 μg/mL.<br><br>Green: CIDE-A Antibody (2089) <br> Blue: DAPI staining
Immunohistochemistry of CIDE-A in human brain tissue with CIDE A antibody at 2.5 μg/mL.
Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.
Trial Size

CIDE-A Antibody

CATALOG NUMBER: 2089

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
CIDE-A antibody was raised against an 18 amino acid peptide near the carboxy terminus of mouse CIDE-A.
The immunogen is located within the last 50 amino acids of CIDE-A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CIDE-A antibody can be used for detection of CIDE-A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
It has no cross activity to CIDE-B.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Predicted Molecular Weight:
25 kDa
Purification:
CIDE-A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CIDE-A antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CIDEA
Additional Names:
CIDE-A Antibody: CIDE-A, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A
Protein Accession Number:
NP_031728
Protein Gi Number:
3114595
Ncbi Gene Id Number:
1149
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CIDE-A Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.
Background Reference 1:
Inohara N, Koseki T, Chen S, et al. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
Background Reference 2:
Inohara N, Koseki T, Chen S, et al. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4 (RD1299)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2089

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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