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Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution.
Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.
Immunofluorescence of DFF40 in Jurkat cells with DFF40 antibody at 20 μg/mL.
Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution.
Trial Size

DFF40 Antibody

CATALOG NUMBER: 2153

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
DFF40 antibody was raised against a 16 amino acid peptide near the amino terminus of human DFF40.
The immunogen is located within the first 50 amino acids of DFF40.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DFF40 antibody can be used for detection of DFF40 by Western blot at 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
40 kDa
Purification:
DFF40 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
DFF40 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Dffb
Additional Names:
DFF40 Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 5730477D02Rik, Cad, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, CAD
Protein Accession Number:
NP_031885
Protein Gi Number:
160948620
Ncbi Gene Id Number:
13368
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Background Reference 1:
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
Background Reference 2:
Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
Background Reference 3:
Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40
Background Reference 4:
Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2153

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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