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Western blot analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 μg/mL.
Immunofluorescence of Bmf in HeLa cells with Bmf antibody at 5 μg/mL.<br><br>Red: Bmf Antibody (3031) <br> Blue: DAPI staining
Western blot analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

Bmf Antibody

CATALOG NUMBER: 3031

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (92%)
Immunogen:
Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf.
The immunogen is located within the first 50 amino acids of Bmf.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 20 kDa
Observed: 22 kDa
Purification:
Bmf Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bmf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bmf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BMF
Additional Names:
Bmf Antibody: Bcl-2-modifying factor
Protein Accession Number:
NP_277038
Protein Gi Number:
15723378
Ncbi Gene Id Number:
90427
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Background Reference 1:
Puthalakath H, Villunger A, O'Reilly LA, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 2001; 293:1829-32.
Background Reference 2:
Hunt A and Evan G. Apoptosis. Till death us do part. Science 2001; 293:1784-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3031

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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