Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 μg/ml.
Immunohistochemical staining of human stomach using TRIM5 alpha antibody at 2 μg/mL.
Immunofluorescence of TRIM5 alpha in Human Stomach cells with TRIM5 alpha antibody at 10 μg/mL.
Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 μg/ml.
Trial Size

TRIM5 alpha Antibody

CATALOG NUMBER: 3247

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of human TRIM5 alpha.
The immunogen is located within amino acids 370 - 420 of TRIM5 alpha.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. A band at approximately 55 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2:
Cat. No. 1317 - Human Uterus Tissue Lysate
Positive Control 3:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 4:
Cat. No. 10-801 - Human Stomach Tissue Slide
Purification:
TRIM5 alpha Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM5 alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM5
Additional Names:
TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88
Protein Accession Number:
NP_149023
Protein Gi Number:
14719418
Ncbi Gene Id Number:
85363
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
Background Reference 1:
Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
Background Reference 2:
Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
Background Reference 3:
Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3247

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369