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Western blot analysis of TLR11 in RAW264.7 cell lysates with TLR11 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemical staining of RAW264.7 cells using TLR11 antibody at 2 μg/mL.
Immunofluorescence of TLR11 in Raw264.7 cells with TLR11 antibody at 10 μg/mL.
Western blot analysis of TLR11 in RAW264.7 cell lysates with TLR11 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TLR11 Antibody

CATALOG NUMBER: 3277

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
TLR11 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of mouse TLR11.
The immunogen is located within the last 50 amino acids of TLR11.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TLR11 antibody can be used for detection of TLR11 by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Positive Control 2:
Cat. No. 17-203 - RAW 264.7 Cell Slide
Purification:
TLR11 Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TLR11 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TLR11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Tlr11
Additional Names:
TLR11 Antibody: Gm287, Gm287, Toll-like receptor 11
Protein Accession Number:
NP_991388
Protein Gi Number:
45429992
Ncbi Gene Id Number:
239081
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TLR11 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR11 is one of three mouse TLRs that lack a human ortholog. It is activated specifically by uropathogenic bacteria, and mice lacking TLR11 showed a much greater susceptability to uropathogenic infections, indicating a potentially important role for TLR11 in preventing infections in the urogenital system.
Background Reference 1:
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Background Reference 2:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 3:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 4:
O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3277

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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