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Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at  1 μg/ml.
Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.
Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at  1 μg/ml.
Trial Size

SIGIRR Antibody

CATALOG NUMBER: 3367

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
SIGIRR antibody was raised against a synthetic peptide corresponding to 15 amino acids at the C-terminus of human SIGIRR.
The immunogen is located within the last 50 amino acids of SIGIRR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1219 – MCF7 Cell Lysate
Positive Control 2:
Cat. No. 17-003 - A549 Cell Slide
Predicted Molecular Weight:
Predicted: 45 kDa
Observed: 45 kDa 
Purification:
SIGIRR Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIGIRR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIGIRR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIGIRR
Additional Names:
SIGIRR Antibody: TIR8, Single Ig IL-1-related receptor, Single Ig IL-1R-related molecule, TIR8
Protein Accession Number:
CAG33619
Protein Gi Number:
48146793
Ncbi Gene Id Number:
59307
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIGIRR Antibody: SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily. Members of this family are defined by the presence of an intracellular Toll-IL-1R (TIR) domain. The Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SIGIRR was originally identified through database analysis and was shown to have only one Ig domain as opposed to the normal three Ig folds seen in the TIR family. Similar to ST2, another TIR family member, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor signaling. However, SIGIRR inhibits TLR-IL-1R signaling by dimerizing with TLR4, TLR5, TLR9, and IL-1R. It also associates with the down-stream TLR signaling proteins IRAK and TRAF6 in an IL-1-dependent fashion.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 3:
Thomassen E, Renshaw BR, and Sims JE. Identification and characterization of SIGIRR, a molecule representing a novel subtype of the IL-1R superfamily. Cytokine 1999; 11:389-99.
Background Reference 4:
Wald D, Qin J, Zhao Z, et al. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat. Immunol. 2003; 4:920-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3367

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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