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Western blot analysis of Lano expression in A431 cell lysate with Lano antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 μg/mL.
Immunofluorescence of Lano in human colon tissue with Lano antibody at 20 μg/mL.
Western blot analysis of Lano expression in A431 cell lysate with Lano antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

Lano Antibody

CATALOG NUMBER: 3001

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (87%)
Immunogen:
Lano antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Lano.
The immunogen is located within the last 50 amino acids of Lano.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Lano antibody can be used for detection of Lano by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1202 – A431 Cell Lysate
Positive Control 2:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 59 kDa
Observed: 59 kDa
Purification:
Lano Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Lano Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Lano antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LRRC1
Additional Names:
Lano Antibody: LANO, dJ523E19.1, LANO, Leucine-rich repeat-containing protein 1, LANO adapter protein
Protein Accession Number:
AAK72246
Protein Gi Number:
14701834
Ncbi Gene Id Number:
55227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Lano Antibody: Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble. The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells. Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions. It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues.
Background Reference 1:
Saito H, Santoni M-J, Jaulin-Bastard F, et al. Lano, a novel LAP protein directly connected to MAGUK proteins in epithelial cells. J. Biol. Chem. 2001; 276:32051-5.
Background Reference 2:
Legouis R, Jaulin-Bastard F, Schott S, et al. Basolateral targeting by leucine-rich repeat domains in epithelial cells. EMBO Rep. 2003; 4:1096-102.
Background Reference 3:
Funke L, Dakoji S, Bredt DS. Membrane-Associated Guanylate Kinases Regulate Adhesion and Plasticity at Cell Junctions. Annu. Rev. Biochem. 2005; 74:219-45.
Background Reference 4:
Bilder D and Perrimon N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 2000; 403:676-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3001

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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