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Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.
Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/mL.
Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.
Trial Size

APOBEC3G Antibody

CATALOG NUMBER: 3257

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G.
The immunogen is located within the first 50 amino acids of APOBEC3G.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
APOBEC3G antibody will also detect the APOBEC3F isoform.
Positive Control 1:
Cat. No. 1223 - Caco-2 Cell Lysate
Positive Control 2:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 3:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
APOBEC3G Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
APOBEC3G Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
APOBEC3G antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
APOBEC3G
Additional Names:
APOBEC3G Antibody: A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1APOBEC-related cytidine deaminase, APOBEC-related protein
Protein Accession Number:
NP_068594
Protein Gi Number:
13399304
Ncbi Gene Id Number:
60489
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
APOBEC3G Antibody: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
Background Reference 1:
Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
Background Reference 2:
Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
Background Reference 3:
Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
Background Reference 4:
Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3257

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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