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Western blot analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemistry of KLHL1 in rat brain tissue with KLHL1 antibody at 10 μg/mL.
Western blot analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

KLHL1 Antibody

CATALOG NUMBER: 3015

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
KLHL1 antibody was raised against a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1.
The immunogen is located within the first 50 amino acids of KLHL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 82 kDa
Observed: 82 kDa
Purification:
KLHL1 Antibody is immunoaffinity purified .
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KLHL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KLHL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLHL1
Additional Names:
KLHL1 Antibody: MRP2, KIAA1490, Kelch-like protein 1
Protein Accession Number:
AAF81719
Protein Gi Number:
8926179
Ncbi Gene Id Number:
57626
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KLHL1 Antibody: The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.
Background Reference 1:
Nemes JP, Benzo KA, and Koob MD. The SCA8 transcript is an antisense RNA to a brain-specific transcript encoding a novel actin-binding protein (KLHL1). Human Mol. Gen. 2000; 9:1543-51.
Background Reference 2:
Adams J, Kelso R, and Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 2000; 10:17-24.
Background Reference 3:
Robinson DN and Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J. Cell Biol. 1997; 138:799-810.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3015

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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