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<strong>Figure 1 KO Validation of ATF6 </strong><br>Loading: 15 μg of human HeLa cell lysate Antibodies ATF6 3683, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Human Cell Lines </strong><br>Loading: 15 μg of human cell lysate Antibodies ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Mouse Tissue </strong><br>Loading: 15 μg of mouse tissue lysate Antibodies: ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Rat Heart </strong><br>Loading: 15 μg of rat heart lysate Antibodies: ATF6 3683, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunohistochemistry Validation of ATF6 in Human Testis Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of ATF6 in Mouse Kidney Tissue </strong><br>Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of ATF6 in Rat Kidney Tissue </strong><br>Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody (3683) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 KO Validation of ATF6
Loading: 15 μg of human HeLa cell lysate Antibodies ATF6 3683, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
KO
Trial Size

ATF6 Antibody

CATALOG NUMBER: 3683

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.
The immunogen is located within amino acids 30 - 80 of ATF6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 0.5-2 μg/mL; IHC: 5 μg/mL
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1211 – HepG2 Cell Lysate
Validation:
KO Validation (Figure 1): Anti-ATF6 antibodies (3683) specificity was further verified by ATF6 specific knockout. ATF6 signal was not detected in ATF6 knockout HeLa cells as compared to that in control wild type cells.
Isoforms:
Human ATF6 has one isoform (670aa, 74.6kD). Mouse ATF6 has one isoform (656aa, 72.7kD) and rat ATF6 also has one isoform (656aa, 72.7kD). 3683 can detect human, mouse and rat.
Purification:
ATF6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATF6
Additional Names:
ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
Protein Accession Number:
NP_031374
Protein Gi Number:
56786157
Ncbi Gene Id Number:
22926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATF6 Antibody: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.
Background Reference 1:
Liu CY and Kaufman RJ. J. Cell Sci. 2003; 1861-2.
Background Reference 2:
Haze K, et al. Mol. Cell. Biol. 1999; 10:3787-99.
Background Reference 3:
Little E, et al. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18
Background Reference 4:
Yoshida H, et al. Cell 2001; 107:881-91.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3683

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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