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<strong>Figure 1 Western Blot Validation with Recombinant Protein</strong><br>Loading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, 3751 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL and Lane 4: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.
<strong>Figure 2 Western Blot Validation in Jurkat Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-32, 3751 (A: 2.5 μg/mL and B: 5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-32 recombinant protein per lane.Antibodies: IL-32, 3751 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL, Lane 3: 2 μg/mL and Lane 4: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 17kD.
Trial Size

IL-32 Antibody

CATALOG NUMBER: 3751

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Anti-IL-32 antibody (3751) was raised against a peptide corresponding to 16 amino acids near the center of human IL-32.
The immunogen is located within amino acids 50 - 100 of IL-32.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5-5 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
This antibody detects the largest isoform of IL-32.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Predicted: 26kD
Observed: 26 kD
Validation:

Recombinant Protein Test (Figure 1): Anti-IL-32 antibodies (3751) detected human IL-32 recombinant protein at different concentrations.

Isoforms:
Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27kD), isoform 2 (188aa, 22kD), isoform 3 (178aa, 21kD), isoform 4 (131aa, 15kD), isoform 5 (168aa, 19kD), isoform 6 (179aa, 21kD) and isoform 7 (148aa, 17kD). 3751 can detect all human isoforms except isoforms 5 and 7.
Purification:
IL-32 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-32 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-32 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL32
Additional Names:
IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32
Protein Accession Number:
AAH09401
Protein Gi Number:
14424787
Ncbi Gene Id Number:
9235
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
Background Reference 1:
Dahl et al. J. Immunol. 1992; 148:597-603.
Background Reference 2:
Kim et al. Immunity 2005; 22:131-42.
Background Reference 3:
Cagnard et al. Eur. Cyto. Network 2005; 16:289-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3751

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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