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Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 μg/mL.
Western blot analysis of PAK6 in Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Trial Size

PAK6 Antibody

CATALOG NUMBER: 3073

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
PAK6 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK6.
The immunogen is located within amino acids 260 - 310 of PAK6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PAK6 antibody can be used for the detection of PAK6 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PAK6 antibody is predicted to not cross-react with other PAK family proteins.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 17-007 - Raji Cell Slide
Predicted Molecular Weight:
Predicted: 75 kDa
Observed: 70 kDa
Purification:
PAK6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PAK6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PAK6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAK6
Additional Names:
PAK6 Antibody: PAK5, PAK5, PAK-5, PAK-6
Protein Accession Number:
NP_064553
Protein Gi Number:
9910476
Ncbi Gene Id Number:
56924
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAK6 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
Background Reference 1:
Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
Background Reference 2:
Yang F, Li X, Sharma M, et al. Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6. J. Biol. Chem. 2001; 276:15345-53.
Background Reference 3:
Kaur R, Liu X, Gjoerup O, et al. Activation of p21-activated kinase 6 by MAP kinase kinase 6 and p38 MAP kinase. J. Biol. Chem. 2005; 280:3323-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3073

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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