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Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.
Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.
Trial Size

Hrk Antibody

CATALOG NUMBER: 3771

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk.
The immunogen is located within the last 50 amino acids of Hrk.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1414 - Mouse Pancreas Tissue Lysate
Predicted Molecular Weight:
Predicted: 10 kDa
Observed: 12 kDa
Purification:
Hrk Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Hrk Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Hrk antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HRK
Additional Names:
Hrk Antibody: DP5, HARAKIRI, BID3, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3
Protein Accession Number:
NP_003797
Protein Gi Number:
4504493
Ncbi Gene Id Number:
8739
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
Background Reference 1:
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Background Reference 2:
Imaizumi K, Tsuda M, Imai Y, et al. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. J. Biol. Chem. 1997; 272:18842-8.
Background Reference 3:
Inohara N, Ding L, Chen S, et al. harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-XL. EMBO J. 1997; 16:1686-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3771

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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