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<strong>Figure 1 Western Blot Validation in Human Daudi Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-27 3799, (A: 2 μg/mL, B: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IL-27 3797 (4 μg/mL), IL-27 3799 (4 µg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Recombinant Protein Validation </strong><br>Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.Antibodies: IL-27 3799, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 μg/mLLane 2: 2 μg/mLLane 3: 4 μg/mL
Figure 1 Western Blot Validation in Human Daudi Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: IL-27 3799, (A: 2 μg/mL, B: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Green Product
Trial Size

IL-27 Antibody

CATALOG NUMBER: 3799

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (92%), Rat: (79%)
Immunogen:
Anti-IL-27 antibody (3799) was raised against a peptide corresponding to 14 amino acids near the center of human IL-27.
The immunogen is located within amino acids 80 - 130 of IL-27.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 2-4 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-207 - EL4 Cell Slide
Positive Control 3:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 5:
Cat. No. 1220 - SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 28 kDa
Observed: 28 kDa
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar IL-27 expression profile in human and mouse cell lines detected by two independent anti-IL-27 antibodies that recognize different epitopes, 3797 against N-terminus domain and 3799 against central domain.  IL-27 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 3) shows that anti-IL-27 antibody (3799) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 3799 antigenic peptide.

Isoforms:
Human IL-27 has one isoform (243aa, 28kD). Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD). 3797 can detect human, mouse and rat IL-27.
Purification:
IL-27 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IL-27 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-27 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL27
Additional Names:
IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha
Protein Accession Number:
NP_663634
Protein Gi Number:
28416913
Ncbi Gene Id Number:
246778
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
Background Reference 1:
Pfanz et al. Immunity 2002; 16:779-90.
Background Reference 2:
Pfanz et al. J. Immunol. 2004; 172:2225-31.
Background Reference 3:
Hisada et al. Cancer Res. 2004; 64:1152-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3799

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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