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Western blot analysis of MD-1 in Daudi cell lysate with MD-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of MD-1 in human spleen tissue with MD-1 antibody at 2 μg/mL.
Western blot analysis of MD-1 in Daudi cell lysate with MD-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

MD-1 Antibody

CATALOG NUMBER: 3847

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (75%)
Immunogen:
MD-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human MD-1.
The immunogen is located within the last 50 amino acids of MD-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
MD-1 antibody can be used for the detection of MD-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
MD-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MD-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LY86
Additional Names:
MD-1 Antibody: MD-1, MMD-1, dJ80N2.1, MD1, Lymphocyte antigen 86, Protein MD-1, Ly-86
Protein Accession Number:
NP_004262
Protein Gi Number:
4758708
Ncbi Gene Id Number:
9450
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MD-1 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins such as RP105, a recently identified homolog of TLR4. This protein, in association with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. While MD-2 can directly bind to LPS, one of the activating molecules of TLR4, the function of MD-1 is less well-known. It has been suggested however, that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Divanovic S, Trompette A, Atabani SF, et al. Inhibition of TLR-4/MD-2 signaling by RP105/MD-1. J. Endotoxin Res. 2005; 11:363-8.
Background Reference 3:
Tsuneyoshi N, Fukudome K, Kohara J, et al. The functional and structural properties of MD-2 required for liposaccharide binding are absent in MD-1. J. Immunol. 2005; 174:340-4.
Background Reference 4:
Nagai Y, Kobayashi T, Motoi Y, et al. The radioprotective 105/MD-1 complex links TLR2 and TLR4/MD-2 in antibody response to microbial membranes. J. Immunol. 2005; 174:7043-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3847

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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