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Western blot analysis of Lipe in human lymph node tissue lysate with Lipe antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of lipe in human lymph node tissue with lipe antibody at 2.5 μg/mL.
Immunofluorescence of Lipe in Human Lymph Node cells with Lipe antibody at 20 μg/mL.
Western blot analysis of Lipe in human lymph node tissue lysate with Lipe antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

Lipe Antibody

CATALOG NUMBER: 3965

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%)
Immunogen:
Lipe antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe.
The immunogen is located within amino acids 920 - 970 of Lipe.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of Lipe are known to exist.
Positive Control 1:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 2:
Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
Predicted Molecular Weight:
Predicted: 85, 90, 110, 118 kDa
Observed: 90 kDa
Purification:
Lipe Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Lipe Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Lipe antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LIPE
Additional Names:
Lipe Antibody: HSL, LHS, Hormone-sensitive lipase, HSL
Protein Accession Number:
ABA03168
Protein Gi Number:
896474
Ncbi Gene Id Number:
3991
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Lipe Antibody: Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.
Background Reference 1:
Yeaman SJ. Hormone-sensitive lipase - new roles for an old enzyme. Biochem. J. 2004; 379:11-22.
Background Reference 2:
Holst LS, Langin D, Mulder H, et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 1996; 35:441-7.
Background Reference 3:
Kraemer FB, Shen WJ, Harada K, et al. Hormone-sensitive lipase is required for high-density lipoprotein cholesterol ester-supported adrenal steroidogenesis. Mol. Endocrinol. 2004; 18:549-57.
Background Reference 4:
Sekiya M, Osuga J, Okazaki H, et al. Absence of hormone-sensitive lipase inhibits obesity and adipogenesis in Lep ob/ob mice. J. Biol. Chem.2004; 279:15084-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3965

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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