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Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 μg/mL.
Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 μg/mL.
Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SnoN Antibody

CATALOG NUMBER: 2255

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN.
The immunogen is located within the first 50 amino acids of SnoN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Purification:
SnoN Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SnoN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
SnoN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SKIL
Additional Names:
SnoN Antibody: SNO, SnoA, SnoI, SnoN, SNO, Ski-like protein, Ski-related oncogene
Protein Accession Number:
NP_005405
Protein Gi Number:
223029418
Ncbi Gene Id Number:
6498
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SnoN Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).
Background Reference 1:
Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
Background Reference 2:
Li Y, Turck CM, Teumer JK, et al. Unique sequence, Ski, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
Background Reference 3:
Luo K. Ski and SnoN: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
Background Reference 4:
Massague J and Wotton D. Transcriptional control by the TGF-β/Smad signaling system. EMBO J. 2000; 19:1745-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2255

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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