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Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of Parc in Daudi cells with Parc antibody at 1 μg/mL.
Immunofluorescence of Bcl-G in Daudi cells with Bcl-G antibody at 2 μg/mL.
Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PARC Antibody

CATALOG NUMBER: 3171

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
PARC antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PARC.
The immunogen is located within the last 50 amino acids of PARC.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
PARC Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PARC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PARC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CUL9
Additional Names:
PARC Antibody: PARC, H7AP1, KIAA0708, PARC, Cullin-9, UbcH7-associated protein 1, CUL-9
Protein Accession Number:
NP_055904
Protein Gi Number:
24307991
Ncbi Gene Id Number:
23113
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PARC Antibody: The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.
Background Reference 1:
Nikolaev AY, Li M, Puskas N, et al. Parc: a cytoplasmic anchor for p53. Cell 2003; 112:29-40.
Background Reference 2:
Skaar JR, Arai T, and DeCaprio JA. Dimerization of CUL7 and PARC is not required for all CUL7 functions and mouse development. Mol. Cell. Biol. 2005; 25:5579-89.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3171

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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