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Western blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 μg/mL.
Immunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 antibody at 10 μg/mL.
Immunofluorescence of p53AIP1 in HL60 cells with p53AIP1 antibody at 20 μg/mL.
Western blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 μg/mL.
Trial Size

p53AIP1 Antibody

CATALOG NUMBER: 2449

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
p53AIP1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human p53AIP1.
The immunogen is located within the first 50 amino acids of p53AIP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
p53AIP1 antibody can be used for detection of p53AIP1 by Western blot at 4 - 8 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1209 - HL60 Cell Lysate
Positive Control 2:
Cat. No. 17-009 - HL-60 Cell Slide
Purification:
p53AIP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
p53AIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
p53AIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TP53AIP1
Additional Names:
p53AIP1 Antibody: P53AIP1, p53-regulated apoptosis-inducing protein 1, p53AIP1
Protein Accession Number:
BAB16421
Protein Gi Number:
10798768
Ncbi Gene Id Number:
63970
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
p53AIP1 Antibody: The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.
Background Reference 1:
Kern SE, Pietenpol JA, Thiagalingam S, Seymour A, et al. Oncogenic forms of p53 inhibit p53-regulated gene expression. Science1991; 252:1708-11.
Background Reference 2:
Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell2000; 102:849-52.
Background Reference 3:
Okamura S, Arakawa H, Tanaka T, et al. p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis. Mol. Cell2001; 8:85-94.
Background Reference 4:
Yoshida K, Monden M, Nakamura Y, et al. Adenovirus-mediated p53AIP1 gene transfer as a new strategy for treatment of p53-resistant tumors. Cancer Sci.2004; 95:91-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2449

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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