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Western blot analysis of TSLP Receptor in mouse heart tissue lysate with TSLP Receptor antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.
Immunofluorescence of TSLP Receptor in Mouse Liver cells with TSLP Receptor antibody at 20 μg/mL.
Western blot analysis of TSLP Receptor in mouse heart tissue lysate with TSLP Receptor antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.
Trial Size

TSLP Receptor Antibody

CATALOG NUMBER: 4209

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
TSLP receptor antibody was raised against a 21 amino acid synthetic peptide from near the center of mouse TSLP receptor.
The immunogen is located within amino acids 140 - 190 of TSLP Receptor.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TSLP receptor antibody can be used for detection of TSLP receptor by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
TSLP Receptor Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TSLP Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TSLP Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Crlf2
Additional Names:
TSLP Receptor Antibody: CRLM2, Ly114, Tpte2, Tslpr, Crlm2, Cytokine receptor-like factor 2, Cytokine receptor-like molecule 2, CRLM-2
Protein Accession Number:
NP_057924
Protein Gi Number:
31980663
Ncbi Gene Id Number:
57914
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSLP Receptor Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells; co-transfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex. This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1. The approximately 75 kDa band seen in the immunoblot may be a post-translationally modified form of TSLP-R.
Background Reference 1:
Ziegler SF and Liu Y-J. Thymic stromal lymphopoietin in normal and pathogenic T cell development and function. Nature Immunol.2006; 7:709-14.
Background Reference 2:
Sims JE, Williams DE, Morrissey PJ, et al. Molecular cloning and biological characterization of a novel murine lymphoid growth factor. J. Exp. Med.2000; 192:671-80.
Background Reference 3:
Levin SD, Koelling RM, Friend SL, et al. Thymic stromal lymphopoietin: a cytokine that promotes the development of IgM+ cells in vitro and signals via a novel mechanism. J. Immunol.1999; 162:677-83.
Background Reference 4:
Park LS, Martin U, Garka K, et al. Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. J. Exp. Med.2000; 192:659-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4209

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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