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Western blot analysis of TRIM25 in HeLa cell lysate with TRIM25 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 5 μg/mL.
Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 μg/mL.
Western blot analysis of TRIM25 in HeLa cell lysate with TRIM25 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TRIM25 Antibody

CATALOG NUMBER: 4317

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (80%)
Immunogen:
TRIM25 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TRIM25.
The immunogen is located within amino acids 40 - 90 of TRIM25.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
TRIM25 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM25 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM25
Additional Names:
TRIM25 Antibody: EFP, Z147, RNF147, ZNF147, EFP, Estrogen-responsive finger protein
Protein Accession Number:
NP_005073
Protein Gi Number:
68160937
Ncbi Gene Id Number:
7706
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
Background Reference 1:
Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA1993; 11117-20.
Background Reference 2:
Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA1999; 96:12027-32.
Background Reference 3:
Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer2001; 91:152-8.
Background Reference 4:
Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature2002; 417:871-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4317

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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