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<strong>Figure 1 Western Blot Validation in Mouse Brain Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: NPTX2 4573 (A: 0.5 μg/mL, B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: NPTX2 4573 (2 μg/mL), NPTX2 64-234 (4 μg/mL), and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Brain Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: NPTX2 4573 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.A: Absence of blocking peptide B: Presence of blocking peptide.
<strong>Figure 4 Immunofluorescence Validation of NPTX2 in Human Brain Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with 4573 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 5 Immunohistochemistry Validation of NPTX2 in Human Brain</strong><br> Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (4573) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of NPTX2 in Mouse Brain Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with 4573 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 7 Immunohistochemistry Validation of NPTX2 in Mouse Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (4573) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation in Mouse Brain Tissue Lysate
Loading: 15 μg of lysates per lane.Antibodies: NPTX2 4573 (A: 0.5 μg/mL, B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

NPTX2 Antibody

CATALOG NUMBER: 4573

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
Immunogen:
Anti-NPTX2 antibody (4573) was raised against a peptide corresponding to 16 amino acids near the center of human NPTX2.
The immunogen is located within amino acids 170 - 220 of NPTX2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.5-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 1201 – HeLa Cell Lysate
Positive Control 3:
Cat. No. 1211 – HepG2 Cell Lysate
Positive Control 4:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 47kD
Observed: 47 kD
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar NPTX2 expression profile in human and mouse cell lines detected by two independent anti-NPTX2 antibodies that recognize different epitopes, 4573 against central domain and 64-234 against another central domain. NPTX2 proteins are detected only in HeLa and HepG2 cell lines by the two independent antibodies.

Isoforms:
Human NPTX2 has one isoform (431aa, 47kD). Mouse NPTX2 has one isoform (429aa, 47.1kD) and Rat NPTX2 also has one isoform (432aa, 47.4kD). 4573 can detect human, mouse and rat.
Purification:
NPTX2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NPTX2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NPTX2
Additional Names:
NPTX2 Antibody: NP2, NARP, NP-II, Neuronal pentraxin-2, Neuronal pentraxin II, NP2
Protein Accession Number:
NP_002514
Protein Gi Number:
28195384
Ncbi Gene Id Number:
4885
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NPTX2 Antibody: Neuronal pentraxin (NPTX) 2 was initially identified as a member of the pentraxin family having high homology to NPTX1. Unlike NPTX1 however, NPTX2 is expressed in testes, pancreas, liver, heart, skeletal muscle as well as brain. NPTX2 possesses the lectin properties common to the pentraxin family, promotes neurite outgrowth, and is rapidly regulated by neuronal activity. NPTX1 and NPTX2 form heterocomplexes that contribute to both activity-independent and -dependent excitatory synaptogenesis. Recently, NPTX2 has been found to be highly upregulated in Parkinsonian substantia nigra and localizes to Lewy bodies and Lewy neurites in sporadic Parkinson's disease (PD), suggesting that it is involved in the pathway dysregulation that underlies PD. This NPTX2 antibody is predicted to be specific to NPTX2 and not recognize NPTX1.
Background Reference 1:
Hsu and Perin. Genomics1995; 28:220-7.
Background Reference 2:
Tsu et al. J. Neurosci.1996; 16:2463-78.
Background Reference 3:
Xu et al. Neuron2003; 39:513-28.
Background Reference 4:
Moran et al. Acta Neuropathol.2008; 115:471-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4573

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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