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Western blot analysis of SATB2 in A20 cell lysate with SATB2 antibody at (A) 2 and (B) 4 μg/mL.
Immunohistochemistry of SATB2 in human brain with SATB2 antibody at 5 μg/mL.
Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 50 μg/mL.
Western blot analysis of SATB2 in A20 cell lysate with SATB2 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

SATB2 Antibody

CATALOG NUMBER: 4629

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SATB2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human SATB2.
The immunogen is located within amino acids 570 - 620 of SATB2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SATB2 antibody can be used for detection of SATB2 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SATB2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SATB2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SATB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SATB2
Additional Names:
SATB2 Antibody: KIAA1034, DNA-binding protein SATB2, Special AT-rich sequence-binding protein 2
Protein Accession Number:
Q9UPW6
Protein Gi Number:
13634020
Ncbi Gene Id Number:
23314
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SATB2 Antibody: Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development.
Background Reference 1:
Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol.1995; 15:456-65.
Background Reference 2:
Szemes M, Gyorgy A, Pawaletz C, et al. Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain. Neurochem. Res.2006; 31:237-46.
Background Reference 3:
Dobreva G, Chahour M, Dautzenberg M, et al. SATB2 is a multifunctional determinant of craniofacial patterning and osteoblast differentiation. Cell2006; 125:971-86.
Background Reference 4:
Gyorgy AB, Szemes M, de Jaun Romero C, et al. SATB2 interacts with chromatin-remodeling molecules in differentiating cortical neurons. Eur. J. Neurosci.2008; 27:865-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4629

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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