Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.
Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.
Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.
Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Wnt10a Antibody

CATALOG NUMBER: 4639

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a.
The immunogen is located within amino acids 300 - 350 of Wnt10a.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This Wnt10a antibody is predicted to not cross-react with Wnt10b.
Positive Control 1:
Cat. No. 1283 - RAW 264.7 Cell Lysate
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 46 kDa
Purification:
Wnt10a Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Wnt10a Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Wnt10a antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WNT10A
Additional Names:
Wnt10a Antibody: OODD, SSPS, STHAG4, Protein Wnt-10a
Protein Accession Number:
Q9GZT5
Protein Gi Number:
14424011
Ncbi Gene Id Number:
80326
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Wnt10a Antibody: Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.
Background Reference 1:
Kelly GM, Lai CJ, and Moon RT. Expression of wnt10a in the central nervous system of developing zebrafish. Dev. Biol. 1993; 158:113-21.
Background Reference 2:
Nusse R and Varmus RE. Wnt genes. Cell 1992; 69:1073-87.
Background Reference 3:
Kirikoshi H, Sekihara H, and Katoh M. WNT10A and WNT6, clustered in human chromosome 2q35 region head-to-tail manner, are strongly coexpressed in SW480 cells. Biochem. Biophys. Res. Commun. 2001; 283:798-805.
Background Reference 4:
Kirikoshi H, Inoue S, Sekihara H, et al. Expression of WNT10A in human cancer. Int. J. Oncol. 2001; 19:997-1001.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4639

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369