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Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.
Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.
Trial Size

Bim Antibody [1C2C8]

CATALOG NUMBER: PM-4819

Clonality:
Monoclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Bim antibody can be used for detection of Bim by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 95-107 - Bim Recombinant Protein
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
Bim Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
Bim Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bim monoclonal antibody can be stored at -20°C, stable for one year.
Ncbi Official Symbol:
BCL2L11
Additional Names:
Bim Antibody [1C2C8] : BAM, BIM, BOD
Protein Accession Number:
NP_619527
Protein Gi Number:
20336315
Ncbi Gene Id Number:
10018
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bim Monoclonal Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Background Reference 1:
O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
Background Reference 2:
Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-4819

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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