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Western blot analysis of NUP107 in A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of NUP107 in A549 cells with NUP107 antibody at 2.5 μg/mL.
Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 μg/mL.
Western blot analysis of NUP107 in A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NUP107 Antibody

CATALOG NUMBER: 4701

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (80%)
Immunogen:
NUP107 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NUP107.
The immunogen is located within amino acids 820 - 870 of NUP107.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
NUP107 antibody can be used for detection of NUP107 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 17-003 - A549 Cell Slide
Purification:
NUP107 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NUP107 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NUP107 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NUP107
Additional Names:
NUP107 Antibody: NUP84, Nuclear pore complex protein Nup107, 107 kDa nucleoporin
Protein Accession Number:
P57740
Protein Gi Number:
12230339
Ncbi Gene Id Number:
57122
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NUP107 Antibody: The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.
Background Reference 1:
Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell2006; 125:1041-53.
Background Reference 2:
Boehmer T, Enninga J, Dales S, et al. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA2003; 100:981-5.
Background Reference 3:
Orjalo AV, Arnaoutov A, Shen Z, et al. The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol. Bio. Cell2006; 17:3806-18.
Background Reference 4:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4701

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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