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Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 μg/mL.
Immunofluorescence of DNAL1 in 3T3 cells with DNAL1 antibody at 20 μg/mL.<br><br>Green: DNAL1 Antibody (4733) <br> Blue: DAPI staining
Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DNAL1 Antibody

CATALOG NUMBER: 4733

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
DNAL1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human DNAL1.
The immunogen is located within the last 50 amino acids of DNAL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
DNAL1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DNAL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DNAL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNAL1
Additional Names:
DNAL1 Antibody: CILD16, C14orf168, Dynein light chain 1, axonemal
Protein Accession Number:
NP_113615
Protein Gi Number:
164607156
Ncbi Gene Id Number:
83544
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNAL1 Antibody: DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.
Background Reference 1:
Horvath J, Fliegauf M, Olbrich H, et al. Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients. Am. J. Respir. Cell Mol. Biol.2005; 33:41-7.
Background Reference 2:
Olbrich H, Haeffner K, Kisbert A, et al. Mutations in DNAH5 cause primary ciliary dyskinesia and randomization of left/right asymmetry. Nat. Genet.2002; 30:143-4.
Background Reference 3:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4733

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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