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Western blot analysis of TRIM30 in mouse heart tissue lysate with TRIM30 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TRIM30 in mouse ovary tissue with TRIM30 antibody at 10 μg/mL.
Immunofluorescence of TRIM30 in Human Ovary cells with TRIM30 antibody at 20 μg/mL.
Western blot analysis of TRIM30 in mouse heart tissue lysate with TRIM30 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TRIM30 Antibody

CATALOG NUMBER: 4747

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Human: (80%)
Immunogen:
TRIM30 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of the mouse TRIM30.
The immunogen is located within amino acids 110 - 160 of TRIM30.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TRIM30 antibody can be used for detection of TRIM30 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Predicted Molecular Weight:
Predicted: 107 kDa
Observed: 105 kDa
Purification:
TRIM30 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM30 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM30 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Trim30
Additional Names:
TRIM30 Antibody: Rpt1, Rpt-1, Trim30, Rpt1, Tripartite motif-containing protein 30A, Down regulatory protein of interleukin-2 receptor
Protein Accession Number:
NP_033125
Protein Gi Number:
125347379
Ncbi Gene Id Number:
20128
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM30 Antibody: TRIM30 belongs to a family of the tripartite motif (TRIM) proteins involved in the regulation of cell proliferation, differentiation, development, oncogenesis, apoptosis and antiviral responses. The TRIM protein family is an expanding family of RING ('really interesting new gene') proteins, also known as RBCC proteins as they contain an RBCC motif, which comprises a RING domain, one or two B-boxes and a predicted coiled-coil region. Studies have shown that some TRIM family members are critical to innate immunity; TRIM5, TRIM19 and TRIM25, for example, have been shown to restrict viral infection. A recent study shows that TRIM30 functions as a negative modulator of the TLR signaling pathway, by targeting TAB2 and TAB3, and contributes to the inhibition of TLR-mediated NF-κB activation. The importance of TRIM30 in the attenuation or termination of NF-κB activation suggests that targeting of TAB2 and TAB3 by TRIM30alpha may be a mechanism for modulating many types of immune responses.
Background Reference 1:
Nisole S, Stoye JP, and Saib A. TRIM family proteins: retroviral restriction and antiviral defence. Nat. Rev. Microbiol.2005; 3:799-808.
Background Reference 2:
Sakuma R, Noser JA, Ohmine S, et al. Rhesus monkey TRIM5 alpha restricts HIV-1 production through rapid degradation of viral Gag polyproteins. Nat. Med.2007; 13:631-635. 
Background Reference 3:
Gack MU, Shin YC, Joo CH, et al. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature2007; 446:916-920. 
Background Reference 4:
Patarca R, Freeman GJ, Schwartz J, et al. rpt-1, an intracellular protein from helper/inducer T cells that regulates gene expression of interleukin 2 receptor and human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA1988; 85:2733-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4747

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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