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Western blot analysis of PRTFDC1 in human brain tissue lysate with PRTFDC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Western blot analysis of PRTFDC1 in human brain tissue lysate with PRTFDC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Trial Size

PRTFDC1 Antibody

CATALOG NUMBER: 4775

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (86%), Pig: (86%), Bovine: (86%), Mouse: (86%)
Immunogen:
PRTFDC1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human PRTFDC1.
The immunogen is located within amino acids 90 - 140 of PRTFDC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PRTFDC1 antibody can be used for detection of PRTFDC1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
PRTFDC1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRTFDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRTFDC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRTFDC1
Additional Names:
PRTFDC1 Antibody: HHGP, HHGP, Phosphoribosyltransferase domain-containing protein 1
Protein Accession Number:
NP_064585
Protein Gi Number:
9910262
Ncbi Gene Id Number:
56952
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRTFDC1 Antibody: Phosphoribosyl transferase domain containing 1 (PRTFDC1) is highly homologous to the hypoxanthine phosphoribosyltransferase (HPRT1) and may have arisen from a gene duplication event of a common ancestor gene. Recently, it was shown that CpG islands in the PRTFDC1 promoter could be hypermethylated in ovarian cancers and oral squamous-cell carcinomas (OSCC), leading to gene silencing. Restoration of PRTFDC1 expression in OSCC inhibited cell growth in colony-formation assays, while knockdown of PRTFDC1 expression in OSCC that expressed the gene promoted cell growth. These results suggest that PRTFDC1 can act as a tumor-suppressor gene. At least three isoforms of PRTFDC1 are known to exist.
Background Reference 1:
Nicklas JA. Pseudogenes of the human HPRT1 gene. Environ. Mol. Mutagen2006; 47:212-8.
Background Reference 2:
Keebaugh AC, Sullivan RT, NISC Comparative Sequencing Program, et al. Gene duplication and inactivation in the HPRT gene family. Genomics2007; 89:134-42.
Background Reference 3:
Cai LY, Abe M, Izumi S, et al. Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers. Life Sci.2007; 80:1458-65.
Background Reference 4:
Suzuki E, Imoto I, Pimkhaokham A, et al. PRTFDC1, a possible tumor-suppressor gene, is frequently silenced in oral squamous-cell carcinoma by aberrant promoter hypermethylation. Oncogene2007; 26:7921-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4775

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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