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<strong>Figure 1 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br>Loading: 30 ng of StrepII-tagged recombinant protein per lane.Antibodies: StrepII-tag 4335, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μg/mLLane 2: 0.5 μg/mLLane 3: 1 μg/mL
<strong>Figure 2 Western Blot Validation with StrepII-tagged Recombinant Protein</strong><br>Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein.Antibodies: StrepII-tag 4335 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 Western Blot Validation with StrepII-tagged Recombinant Protein
Loading: 30 ng of StrepII-tagged recombinant protein per lane.Antibodies: StrepII-tag 4335, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μg/mLLane 2: 0.5 μg/mLLane 3: 1 μg/mL
Trial Size

StrepII-tag Antibody

CATALOG NUMBER: 4335

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Tag
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Tag
Immunogen:
Anti-StrepII-tag antibody (4335) StrepII-tag antibody was raised against the amino acid sequence " srWSHPQFEKsh".
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.25-1 μg/mL
Antibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins
Validation:

Recombinant Protein Test (Figure 1&2): Anti-StrepII-tag antibodies (4335) detected StrepII-tagged recombinant protein at different concentrations.

Purification:
StrepII-tag Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
StrepII-tag antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Names:
StrepII-tag Antibody:
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
Background Reference 1:
Schmidt and SKerra. Protein Eng.1993;6:109-22.
Background Reference 2:
Schmidt et al. J. Mol. Biol. 1996;255:753-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4335

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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