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Western blot analysis of Rkhd1 in MDA-MB-361 cell lysate with Rkhd1 antibody at (A) 1 μg/ml and (B) 2 μg/mL.
Immunohistochemistry of Rkhd1 in human small intestine tissue with Rkhd1 antibody at 2.5 μg/mL.
Immunofluorescence of rkhd1 in human small intestine tissue with rkhd1 antibody at 20 μg/mL.
Western blot analysis of Rkhd1 in MDA-MB-361 cell lysate with Rkhd1 antibody at (A) 1 μg/ml and (B) 2 μg/mL.
Trial Size

Rkhd1 Antibody

CATALOG NUMBER: 4835

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (73%)
Immunogen:
Rkhd1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Rkhd1.
The immunogen is located within amino acids 360 - 410 of Rkhd1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Rkhd1 antibody can be used for detection of Rkhd1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1217 - MDA-MB-361 Cell Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Purification:
Rkhd1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Rkhd1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Rkhd1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MEX3D
Additional Names:
Rkhd1 Antibody: MEX3, TINO, RKHD1, MEX-3D, RNF193, OK/SW-cl.4, KIAA2031, RNA-binding protein MEX3D, RING finger and KH domain-containing protein 1
Protein Accession Number:
Q86XN8
Protein Gi Number:
134047829
Ncbi Gene Id Number:
399664
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rkhd1 Antibody: Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3'-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.
Background Reference 1:
Donnini M, Lapucci A, Papucci L, et al. Identification of TINO: A new evolutionarily conserved Bcl-2 AU-rich element RNA-binding protein. J. Biol. Chem.2004; 279:20154-66.
Background Reference 2:
Draper BW, Mello CC, Bowerman B, et al. MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegansembryos. Cell1996; 87:205-16.
Background Reference 3:
Liu J, Valencia-Sanchez MA, Hannon GJ, et al. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol2005; 7:719-23.
Background Reference 4:
Buchet-Poyau K, Courchet J, Le Hir H, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res.2007; 35:1289-300.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4835

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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