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Western blot analysis of Gle1 in mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 μg/mL.
Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 5 μg/mL.
Western blot analysis of Gle1 in mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Gle1 Antibody

CATALOG NUMBER: 4963

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Gle1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Gle1.
The immunogen is located within amino acids 540 - 590 of Gle1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Gle1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Gle1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Gle1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GLE1
Additional Names:
Gle1 Antibody: LCCS, GLE1L, LCCS1, hGLE1, Nucleoporin GLE1, GLE1-like protein
Protein Accession Number:
NP_001003722
Protein Gi Number:
51317384
Ncbi Gene Id Number:
2733
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.
Background Reference 1:
Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature1996; 383:357-60.
Background Reference 2:
Watkins JL, Murphy R, Emtage JL, et al. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export. Proc. Natl. Acad. Sci. USA1998; 95:6779-84.
Background Reference 3:
Kendirgi F, Rexer DJ, Alcazar-Roman AR, et al. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: A conserved mechanism in the export of Hsp70 mRNA. Mol. Biol. Cell2005; 16:4304-15.
Background Reference 4:
Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell. Proteomics2004; 3:145-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4963

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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