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Western blot analysis of OCC-1 in 293 cell lysate with OCC-1 antibody at (A) 2 μg/mL and (B) 4 μg/mL.
Immunohistochemistry of OCC-1 in human spleen tissue with OCC-1 antibody at 2.5 μg/mL.
Western blot analysis of OCC-1 in 293 cell lysate with OCC-1 antibody at (A) 2 μg/mL and (B) 4 μg/mL.
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OCC-1 Antibody

CATALOG NUMBER: 4987

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
OCC-1 antibody was raised against a 7 amino acid synthetic peptide near the carboxy terminus of human OCC-1.
The immunogen is located within the last 50 amino acids of OCC-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
OCC-1 antibody can be used for detection of OCC-1 by Western blot at 2 - 4 μg/mL. In vitro transcription and translation of the OCC-1 cDNA yields polypeptides less than 9 kDa. Despite this, OCC-1 from cell lines is detected at ~28 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
OCC-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
OCC-1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
OCC-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C12orf75
Additional Names:
OCC-1 Antibody: AGD3, OCC1, OCC-1, C12orf75, Overexpressed in colon carcinoma 1 protein, AGD3
Protein Accession Number:
AAH61920
Protein Gi Number:
112180576
Ncbi Gene Id Number:
387882
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OCC-1 Antibody: OCC-1 (Overexpressed in colon carcinoma-1) was initially identified as a novel human cDNA overexpressed in a colon carcinoma cell line. Elevated levels of OCC-1 mRNA were observed in three of eight colon carcinomas, suggesting that OCC-1 may be a hallmark of only a subset of colon carcinomas. It is highly expressed in skeletal muscle, kidney, placenta, and pancreas tissues, with low to no expression in heart, brain, lung and liver tissues. At least two different OCC-1 mRNA species are known to exist.
Background Reference 1:
Pibouin L, Villaudy J, Ferbus D, et al. Cloning of the mRNA of overexpression in colon-carcinoma-1: a sequence overexpressed in a subset of colon carcinomas. Cancer Genet. Cytogenet.2002; 133:55-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4987

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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