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Western blot analysis of GNPDA2 in human kidney lysate with GNPDA2 antibody at 1 μg/mL.
Immunocytochemistry of GNPDA2 in 293 cells with GNPDA2 antibody at 4 μg/mL.
Immunofluorescence of GNPDA2 in mouse kidney tissue with GNPDA2 antibody at 20 μg/mL.
Western blot analysis of GNPDA2 in human kidney lysate with GNPDA2 antibody at 1 μg/mL.
Trial Size

GNPDA2 Antibody

CATALOG NUMBER: 5061

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
GNPDA2 antibody was raised against an 8 amino acid synthetic peptide near the carboxy terminus of human GNPDA2.
The immunogen is located within the last 50 amino acids of GNPDA2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
GNPDA2 antibody is predicted to not cross-react with GNPDA1.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
GNPDA2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GNPDA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GNPDA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GNPDA2
Additional Names:
GNPDA2 Antibody: GNP2, SB52, GNP2, Glucosamine-6-phosphate isomerase 2, Glucosamine-6-phosphate deaminase 2, GNPDA 2
Protein Accession Number:
NP_612208
Protein Gi Number:
19923881
Ncbi Gene Id Number:
132789
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GNPDA2 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA2 is the second mammalian glucosamine-6-phosphate deaminase discovered, and is closer in structure and activity to the E. coli enzyme than GNPDA1. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
Background Reference 1:
Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9.
Background Reference 2:
Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem.2003; 88:932-40.
Background Reference 3:
Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett.2003; 551:63-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5061

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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